You can change the ability by right clicking the button. Dont think you can clear it in game
If you are wanting to clear out a button back to unassigned edit your characters settings file (CharName_P1999.ini in the main EQ folder) and set the Ability#=-1. For example below has Ability1 and Ability2 buttons set and the rest are unassigned
[Abilities]
Ability1=40
Ability2=27
Ability3=-1
Ability4=-1
Ability5=-1
Ability6=-1
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